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KMID : 0881720140290040376
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Journal of Food Hygiene and Safety
2014 Volume.29 No. 4 p.376 ~ p.382
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Antimicrobial Activities of Sword Bean (Canavalia gladiata) Extracts against Food Poisoning Bacteria
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Chung Jae-Keun
Ha Dong-Ryong
Lee Jeong-Chi
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Abstract
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Various solvents (chloroform, hexane, ethyl acetate, ethanol, methanol, and hot water) were tested to investigate the antimicrobial activities of sword bean (Canavalia gladiata) against 12 food poisoning bacteria. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but ethanol extract showed V. parahemolyticus 10 mm, S. sonnei 9 mm, and methanol extract showed strong activities in order of V. parahemolyticus 22 mm, S. sonnei 21 mm, L. monocytogenes 20 mm by disk diffusion. The minimal inhibitory concentrations (MICs) were also determined. The methanol extract had MIC values of 50 mg/mL against S. Typhimurium, V. parahemolyticus, and S. sonnei and values of 100 mg/mL against other 7 food poisoning bacteria and values of 200 mg/ mL against Y. enterocolitica and MRSA. The inhibitory effect of methanol sword bean extract on the growth of V. parahemolyticus was investigated. Growth of the strain occurred at the concentration of 0.5% extract and was inhibited continuously at 1.0 and 1.5% for 30hours after inoculation, whereas the strain was completely inhibited at 2.0% after 9hours of inoculation
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KEYWORD
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sword bean, Canavalia gladiata, antimicrobial activity, MICs, food poisoning
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